Evaluation of Salivary Biomarker Levels of Individuals With Different Periodontal Diseases
Study Details
Study Description
Brief Summary
The aim of this study is; detection of leucine rich α-2 glycoprotein1 (LRG1), c-reactive protein (CRP) levels in saliva samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples of saliva were obtained from 60 systemically healthy non-smoker individuals with periodontitis (P, n=20), gingivitis(G, n=20) and healthy periodontium (S, n=20). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine LRG and CRP levels in the biological samples.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: Healthy Periodontium Full-mouth clinical periodontal measurements recorded and saliva obtained. |
Diagnostic Test: saliva obtaining
The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.
|
| Experimental: Gingivitis Full-mouth clinical periodontal measurements recorded and saliva obtained. |
Diagnostic Test: saliva obtaining
The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.
|
| Experimental: Periodontitis Full-mouth clinical periodontal measurements recorded and saliva obtained. |
Diagnostic Test: saliva obtaining
The patient was asked to sit upright and tilt his/her head forward to collect saliva samples. İn this way, unstimulated saliva was allowed to accumulate in the floor of the mouth. The accumulated saliva was collected in a sterile container. It was then transferred to a propylene tube. The tubes were centrifuged and the clear part at the top of the tube was taken with a sterile syringe and transferred to a different propylene tube with 0.5 ml in each tube. Tubes were stored at -80ºC until the day of analysis.
|
Outcome Measures
Primary Outcome Measures
- The total amount of LRG1 in saliva [24 hours after taking the clinical measurements at the first visit]
The total amount of leucine rich α-2 glycoprotein1 in saliva
- The total amount of CRP in saliva [24 hours after taking the clinical measurements at the first visit]
The total amount of C-reactive protein in saliva
Eligibility Criteria
Criteria
Inclusion Criteria:
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Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
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At least twenty permanent teeth in the mouth
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Non-smoker
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No medication for continuous use
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Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
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Not in pregnancy or lactation period.
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For the periodontitis group that has not received periodontal treatment in the last 6 months
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For the periodontitis group; According to the evaluation made in 6 regions of each tooth, individuals with 30% or more bleeding on probing area, at least 2 teeth not adjacent to each quarter jaw with a depth of 5 mm or more and 4 mm or more attachment loss, coronal 1/3 and more on radiography (horizontal and / or vertical) bone loss
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For the gingivitis group; According to the evaluation made in 6 regions of each tooth, individuals with 10% or more bleeding on probing area, having a probing depth of less than 4mm and no attachment loss
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For healthy group; According to the evaluation made in 6 regions of each tooth,individuals with less than 10% bleeding on probing area, having a probing depth less than 4mm and no loss of attachment were included in the study.
Exclusion Criteria:
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Any oral or systemic disease
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Regularly using a systemic medication
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During pregnancy or lactation
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Received periodontal treatment within the last 6 months.
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Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
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Smokers are not included in the study
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Izmir Katip Çelebi University Department of Periodontology | İzmir | Çiğli | Turkey | 35640 |
Sponsors and Collaborators
- Izmir Katip Celebi University
Investigators
- Study Director: Mehmet Sağlam, Izmir Katip Çelebi University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 2021-TDU-DİŞF-0016