EMTA: Evaluation of the Effects of Immune Cells on Periodontal Healing
Study Details
Study Description
Brief Summary
Periodontitis is an inflammatory disease with an infectious character, where, as the result of the host response to a dysbiotic microflora, attachment and bone loss occur. The host response and the healing period following the treatment differs among individuals, but the reason behind is not fully understood. The macrophages and T cells play an important role in the immune response and in the pathogenesis of periodontal diseases, but their role in the healing following periodontal therapy is not known. In this study, we aim to reveal the effects of initial macrophage and T cell activities in the gingival tissue on the differences of the response to phase I periodontal treatment.
42 individuals will be included in the study. Granulation tissue samples will be collected from two separate deep pockets of each individual, initially. At the same session, full-mouth scaling and root debridement will be conducted. Saliva, subgingival biofilm and gingival crevicular fluid (GCF) samples will also be collected, initially, and at the 2nd, 6th, 12th and 24th weeks. At the same appointments, periodontal parameters will be recorded. When the clinical procedures are concluded, the samples will be sent to Turku University with dry ice. Tissue and GCF concentrations of related cytokines will be analyzed with Luminex. The density of macrophage types will be defined by immunoblot analysis of related markers. Macrophage subpopulations in tissues will be specified by proteomics. Likewise, quantities of periodontal pathogens will be evaluated with DNA isolation and next generation sequencing.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: Periodontitis patients Single-group receiving periodontal treatment. The data will be evaluated according to the healing potential of individuals in the group and also site-specifically. |
Procedure: Initial periodontal treatment
Conventional periodontal treatment (scaling and root debridement) will be conducted
|
Outcome Measures
Primary Outcome Measures
- Change in Probable pocket depth (PPD) [baseline, 2nd, 6th, 12th and 24th weeks following therapy]
distance between the gingival margin and pocket base (mm); full-mouth scores, 6 sites per tooth
- Change in Bleeding on probing (BoP) [baseline, 2nd, 6th, 12th and 24th weeks following therapy]
visual inspection, dichotomous; full-mouth, 6 sites per tooth; change in positive sites %
- Baseline macrophage related cytokine concentrations in granulation tissue samples [Samples will be obtained during the intervention and will be kept in -80oC until immunohistochemical analysis which will be carried out through study completion, 6-9 months]
two pockets (≥6mm; BoP+) of each individual; Luminex: monocyte chemoattractant protein (MCP)-1, -2, -3, -4; macrophage derived chemokine (MDC); macrophage inhibitory factor (MIF), monokine induced by gamma interferon (MIG), macrophage inflammatory protein (MIP)-1α, interferon γ-induced protein (IP)-10, CD163, TWEAK, BAFF concentrations (pg/mL); each will be correlated with the change in probable pocket depth and bleeding on probing scores
- Baseline Th17-pathway related cytokine concentrations in granulation tissue samples [Samples will be obtained during the intervention and will be kept in -80oC until immunohistochemical analysis which will be carried out through study completion, 6-9 months]
two pockets (≥6mm; BoP+) of each individual; Luminex: Interleukin (IL)-1β, -4, -6, -10, -17A, -17F, -21, -22, -23, -25, -31, -33, interferon (IFN)-γ, soluble cluster of differentiation 40 ligand (sCD-40L), tumor necrosis factor (TNF)-ɑ concentrations (pg/mL); each will be correlated with the change in probable pocket depth and bleeding on probing scores
- Baseline density of macrophage types in granulation tissue samples [Samples will be obtained during the intervention and will be kept in -80oC until immunohistochemical analysis which will be carried out through study completion, 6-9 months]
two pockets (≥6mm; BoP+) from each individual; Immunoblot analysis: cluster of differentiation(CD)68 and CD163; cell number / optic field (cells/mm2); each will be correlated with the change in probable pocket depth and bleeding on probing scores
Secondary Outcome Measures
- Change in macrophage and Th17-pathway related cytokine concentrations in saliva [baseline, 2nd, 6th, 12th and 24th weeks following therapy; immunohistochemical analysis will be carried out through study completion, 6-9 months]
MCP-1, -2, -3, -4, MDC, MIF, MIG, MIP-1α, IP-10, IL1β, -4, -6, -10, -17A, -17F, -21, -22, -23, -25, -31, -33, IFN-γ, sCD-40L, TNF-ɑ, sCD163, TWEAK, BAFF, sST2 concentrations (pg/mL); will be correlated with the change in probable pocket depth and bleeding on probing scores
- Change in macrophage and Th17-pathway related cytokine concentrations in gingival crevicular fluid (GCF) [baseline, 2nd, 6th, 12th and 24th weeks following therapy; immunohistochemical analysis will be carried out when through study completion, 6-9 months]
MCP-1, -2, -3, -4, MDC, MIF, MIG, MIP-1α, IP-10, IL1β, -4, -6, -10, -17A, -17F, -21, -22, -23, -25, -31, -33, IFN-γ, sCD-40L, TNF-ɑ, sCD163, TWEAK, BAFF, sST2 concentrations (pg/mL); will be correlated with the change in probable pocket depth and bleeding on probing scores
- Change in plaque index [baseline, 2nd, 6th, 12th and 24th weeks following therapy]
Silness-Löe plaque index (scored as 0-3); full-mouth, 6 sites per tooth; will be monitored for oral health assessment during the healing period
- Change in clinical attachment level (CAL) [baseline, 2nd, 6th, 12th and 24th weeks following therapy]
distance between the cement-enamel junction and pocket base (mm); full-mouth, 6 sites per tooth; will be correlated with pocket depth reduction
- Change in biofilm microbiota [baseline, 2nd, 6th, 12th and 24th weeks following therapy; microbiological analysis will be carried out through clinical phase completion, 6-9 months]
DNA isolation and next generation sequencing: P. gingivalis, T. denticola, T. forsythia, P. intermedia, F. nucleatum and A. actinomycetemcomitans; bacterial count (log10 scale)
- Change in neutrophil-associated cytokines in saliva [baseline, 6th, 12th and 24th weeks following therapy]
MMP-2, MMP-7, MMP-8, active MMP-8, MMP-9, MMP-13, TIMP, myeloperoxidase, PMN elastase (IFMA and ELISA methods) concentrations (pg/mL) in saliva
- Change in neutrophil-associated cytokines in and oral rinse [baseline, 6th, 12th and 24th weeks following therapy]
MMP-2, MMP-7, MMP-8, active MMP-8, MMP-9, MMP-13, TIMP, myeloperoxidase, PMN elastase (IFMA and ELISA methods) concentrations (pg/mL) in oral rinse samples
- active MMP-8 point-of-care test results in oral rinse [baseline, 6th, 12th and 24th weeks following therapy]
Dichotomous aMMP-8 test results in oral rinse samples (according to the manufacturer, a concentration below 20 ng/mL gives a negative test result, otherwise positive - Periosafe (R) Dentognostics GmHb, Jena, Germany)
Eligibility Criteria
Criteria
Inclusion Criteria:
-
moderate to severe periodontitis
-
having at least two pockets ≥ 6 mm
-
systemic healthy
Exclusion Criteria:
-
received periodontal treatment prior to study
-
received antibiotic or antiinflammatory drugs in the last 6 months
-
pregnant or in lactation
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Biruni University | Istanbul | Turkey |
Sponsors and Collaborators
- Biruni University
- The Scientific and Technological Research Council of Turkey
- University of Turku
Investigators
- Principal Investigator: Mustafa YILMAZ, Biruni University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- TheMacPer