The Potential Association of Long Non-coding RNA (NEAT1 and MALAT1) in the Process of Epithelial-mesenchymal Transition in Periodontitis
Study Details
Study Description
Brief Summary
Periodontitis is a polymicrobial, inflammatory condition affecting tooth-supporting tissues. It is characterized by a progressive loss of epithelial attachment and resorption of alveolar bone, which can lead to tooth loss. Epithelial-mesenchymal transition (EMT) has been proposed as a reversible process that shifts the cell phenotype from epithelial to mesenchymal-like and may be involved in the process of periodontal inflammation either completely or partially. Long non-coding RNAs (lncRNAs) are a large class of regulatory transcripts longer than 200 nucleotides lacking evident protein-coding potential. Studies have shown that lncRNA dysregulation plays key roles in human diseases, including cancer, by modulating the epithelial-mesenchymal transition (EMT). The involvement of lncRNAs markers in the pathogenesis of periodontal disease induced EMT has not been studied thoroughly and have created a gap in the knowledge.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| control group -healthy groups/without the condition |
|
| study group -diseased group-/with the condition (periodontitis) |
Other: periodontitis challenge
control will be compared to diseased group suffering from periodontal disease.
|
Outcome Measures
Primary Outcome Measures
- determining the relative expression of lncRNAs (NEAT1 and MALAT1) in healthy and diseased periodontal tissue. [one year]
reverse transcription real time polymerase chain reaction
Secondary Outcome Measures
- determine the expression of EMT-related genes (E-cadherin, Vimentin, β-catenin, and Snail1), in healthy and diseased tissues, and their correlation with lncRNAs and clinical parameters. [one year]
reverse transcription real time polymerase chain reaction
Eligibility Criteria
Criteria
Inclusion Criteria:
-
Patients with no history of systemic disease e.g., diabetes.
-
Both males and females will be included in the study.
-
Control group: patients fit the case definition of gingival health2, and confirmed by Histopathological inflammatory cell infiltration analysis.
-
Periodontitis group: patients fit the case definition of periodontal disease referred for periodontal flap surgery (modified Widman's flap).
-
Patients radiographically confirmed with infrabony pocket eligible for periodontal flap surgery.
Exclusion Criteria:
-
Patients not willing to participate or consent.
-
Patient taking medications that affect or modify immunity (e.g., corticosteroid), for the last 3 months.
-
Patient taking NSAIDs for the last 3 months.
-
Active smoker.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | saif mohammed T AL Mufti | Baghdad | Iraq | 964 |
Sponsors and Collaborators
- Ali A Abdulkareem
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- lncRNA in periodontal disease