Diagnostic Immunization With Rabies Vaccine in Patients With PID

• Sponsor: Chulalongkorn University (Other)
• Overall Status: Unknown status
• CT.gov ID: NCT02490956
• Collaborator: (none)
• Enrollment: 40
• Location: 1
• Arm: 1
• Duration (Months): 17.1
• Patients Per Site Per Month: 2.3

Study Details

Study Description

Brief Summary

The purpose of this study is to evaluate diagnostic immunization protocol of rabies vaccine for diagnosis the patients with primary immunodeficiency disorders and study humoral and cellular immune response to rabies vaccine in patients with primary immunodeficiency.

Condition or Disease	Intervention/Treatment	Phase
Primary Immunodeficiency	Biological: Verorab® (PVRV; Purified Vero Cell Vaccine)	Phase 4

Detailed Description

Objective Primary objective

: To study diagnostic immunization protocol of rabies vaccine for diagnosis the patients with primary immunodeficiency disorders. Secondary objective

• To study humoral and cellular immune response to rabies vaccine in patients with primary immunodeficiency.

• To study anti-rabies immunization protocol in patients with primary immunodeficiency disorders.

Population Case group: Twenty primary immunodeficiency disease patients that are diagnosed, treated and followed at Allergy and Immunology unit, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University.

Control group: Twenty healthy subjects who have no underlying disease and age-matched with case patients. Inclusion and exclusion criteria

Vaccine :

• Verorab® (PVRV; Purified Vero Cell Vaccine) 0.5 ml intramuscular

• Standard intramuscular regimen: ESSEN on days 0, 3, 7, 14, 28 and booster at 1 year later on days 360 and 363

Immunological evaluations :

1. Humoral immune response :

on 5 ml blood samples will be collected for antibody determination days 0, 14, 28, 90, 360, 367 and 374. Neutralizing antibodies will be determined blindly using the rapid fluorescent focus inhibition test (RFFIT) at Queen Saovabha Memorial Institute. Rabies neutralizing antibody will be reported in IU/ml The protective antibody level are defined rabies neutralizing antibody ≥ 0.5 IU/ml .

1. Cellular mediated immune response :

On 5 ml blood samples will be collected for antibody determination days 14, 28, 90, 360, 367 and 374. Lymphocyte proliferation response to rabies antigen will be determined by using 3H-thymidine incorporation assay.

The responder criteria are defined as stimulation index (SI index) ≥ 2.0 was considered as evidence of antigen-induced lymphocyte proliferation.

Study Design

• Study Type: Interventional
• Anticipated Enrollment: 40 participants
• Allocation: N/A
• Intervention Model: Single Group Assignment
• Masking: None (Open Label)
• Primary Purpose: Diagnostic
• Official Title: Diagnostic Immunization With Rabies Vaccine in Patients With Primary Immunodeficiency Disorders
• Study Start Date: Apr 1, 2015
• Anticipated Primary Completion Date: Sep 1, 2016
• Anticipated Study Completion Date: Sep 1, 2016

Arms and Interventions

Arm	Intervention/Treatment
Experimental: Rabies vaccination Verorab® (PVRV; Purified Vero Cell Vaccine) 0.5 ml intramuscular Standard intramuscular regimen: ESSEN on days 0, 3, 7, 14, 28 and booster at 1 year later on days 360 and 363	Biological: Verorab® (PVRV; Purified Vero Cell Vaccine); Verorab® (PVRV; Purified Vero Cell Vaccine) 0.5 ml intramuscular Standard intramuscular regimen: ESSEN on days 0, 3, 7, 14, 28 and booster at 1 year later on days 360 and 363

Outcome Measures

Primary Outcome Measures

1. Rabies neutralizing antibody titer (RFFIT test) [1 year]

Secondary Outcome Measures

1. Lymphocyte proliferation response to rabies antigen (3H-Thymidine incorporation assay) [1 year]

Eligibility Criteria

Criteria

• Ages Eligible for Study: 12 Months to 60 Years
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: Yes

Inclusion Criteria:

• Case group

1. Patient with primary immunodeficiency diseases who follows up at Division of pediatric allergy and immunology unit, King Chulalongkorn Memorial Hospital

2. Subject or father/mother/legally acceptable representative properly informed about the study and having signed the informed consent form.

3. Subject is able to comply with the follow-up schedule of the protocol

• Control group

1. Subject is healthy (from history and physical examination)

2. Subject do not has underlying diseases.

3. Subject do not has primary and secondary immunodeficiency diseases

4. Subject is not receiving immunosuppressive therapy or cytotoxic drugs

5. Subject or father/mother/legally acceptable representative properly informed about the study and having signed the informed consent form.

6. Subject is able to comply with the follow-up schedule of the protocol

7. Age more than 12 month old to 60 years old

Exclusion Criteria:

1. Reported history of previous rabies immunization or Rabies neutralizing antibody (Rabies Nab) > 0.5 IU/ml

2. Subject is unable to comply with the follow-up schedule of the protocol

3. Pregnancy

4. Body temperature more than 38 degree celcius at screening visit

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	Division of Allergic and Immunology , Department of Pediatrics, Faculty of Medicine, Chulalongkorn University	Bangkok		Thailand	10330

Sponsors and Collaborators

• Chulalongkorn University

Investigators

• Principal Investigator: Narissara - Suratannon, MD, Chulalongkorn University

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

• Responsible Party: Narissara Suratannon, Faculty of Medicine, Chulalongkorn university, Chulalongkorn University
• ClinicalTrials.gov Identifier: NCT02490956
• Other Study ID Numbers: RabiesPID
• First Posted: Jul 7, 2015
• Last Update Posted: Jul 7, 2015
• Last Verified: Jul 1, 2015

• Keywords provided by Narissara Suratannon, Faculty of Medicine, Chulalongkorn university, Chulalongkorn University: Diagnostic immunization with rabies vaccine; Primary immunodeficiency
• Additional relevant MeSH terms: Rabies; Primary Immunodeficiency Diseases; Immunologic Deficiency Syndromes

Study Results

No Results Posted as of Jul 7, 2015