PROSECT: Prospective Cohort Study of Changes in Circulatory MicroRNA of Resected Hepatocellular Carcinoma

Sponsor

National Cancer Centre, Singapore (Other)

Overall Status

Recruiting

CT.gov ID

NCT05148572

Collaborator

Duke-NUS Graduate Medical School (Other), Singapore Phenome Centre (Other), Nanyang Technological University (Other), MiRXES Pte Ltd (Other), Asian Microbiome Library (AMiLi) Pte Ltd (Other)

100

Enrollment

Locations

39.4

Anticipated Duration (Months)

16.7

Patients Per Site

0.4

Patients Per Site Per Month

Study Details

Study Description

Brief Summary

Hepatocellular carcinoma (HCC) is the 4th most common cause of cancer death globally but only 20% are diagnosed in its early stages where curative treatment can be carried out. Current standard-of-care surveillance of patients at high risk of developing HCC with 6-monthly serum alpha-fetoprotein (AFP) and ultrasound imaging (US) has a sensitivity of approximately 63% for detecting early HCC. There is an urgent need for a more efficacious and convenient modality of surveillance of high-risk patients to diagnose HCC at an early stage. In another study (AHCC10 ELEGANCE, NCT04965259), 2,000 patients at risk of developing HCC will be enrolled to develop the 1st miRNA in-vitro diagnostic (IVD) kit for HCC that has higher accuracy and better ease of use compared with the extant combination of AFP and US.

This prospective study will act as a positive control to the AHCC10 ELEGANCE Study and aims to address the absence of efficacious modalities of surveillance by validating a panel of circulating miRNA biomarkers signatures with histologically proven HCC. This study will determine progressive changes in the profiles of miRNA signatures pre- and post- surgical resection to identify signatures predictive of recurrence.

Additionally, this study also aims to identify changes in key metabolites and microbiome with correlation to changes in choline, bile acid and tryptophan metabolic pathways with changes in the composition and function of gut microbiota to establish actionable biomarkers that can predict HCC recurrence.

Condition or Disease	Intervention/Treatment	Phase
Hepatocellular Carcinoma

Detailed Description

This is a prospective study involving 100 patients with diagnosis of hepatocellular carcinoma (HCC) confirmed by histology at surgical resection. Eligible patients will receive CT scans at 6 months and 12 months post-surgery to monitor for recurrence. Pre- and post-surgery bio-samples (blood, urine, stool) will also be collected to monitor changes in profiles of miRNA, metabolome and microbiome.

Study Design

Study Type:

Observational

Anticipated Enrollment :

100 participants

Observational Model:

Cohort

Time Perspective:

Prospective

Official Title:

Prospective Cohort Study of Changes in Circulatory MicroRNA After Surgical Resection of Hepatocellular Carcinoma (HCC) (PROSECT)

Actual Study Start Date :

Nov 10, 2021

Anticipated Primary Completion Date :

Feb 21, 2024

Anticipated Study Completion Date :

Feb 21, 2025

Outcome Measures

Primary Outcome Measures

miRNA biomarker signatures diagnostic for HCC or for recurrence prediction. [Baseline and every 6 monthly thereafter, up to 12 months.]
Changes in the profile of circulating micro-RNA biomarkers pre- and post- surgical resection.
Gut microbiome profiling for recurrence prediction. [Baseline.]
Differences in the profile of gut microbiota among HCC and healthy population retrieved from database.
Metabolome profiling for recurrence prediction. [Baseline and every 6 monthly thereafter, up to 12 months.]
Changes in the profile of metabolome in urine and plasma pre- and post- surgical resection.

Eligibility Criteria

Criteria

Ages Eligible for Study:

21 Years to 90 Years

Sexes Eligible for Study:

All

Accepts Healthy Volunteers:

Inclusion Criteria:

The patient is male or female patients, 21 to 90 years of age at the time of signature of the informed consent form.
The patient has clinically AND histologically proven HCC.
The patient has R0 or R1 resection on histology.
The patient has Child-Pugh <= 7 points without clinical ascites before surgery.
The patient has ECOG performance status 0-1 before surgery.
The patient is scheduled for liver resection within 6 weeks of signing screening informed consent form.
The patient has received no anti-cancer specific treatment for HCC before the surgery (eg. previous liver resection, loco-regional therapy such as RFA, TACE, SIRT, radiotherapy, immunotherapy, chemotherapy or neo-adjuvant chemotherapy), other than the planned surgery. However, patient who has received previous HCC treatment more than 5 years ago is deemed to have a de-novo liver tumour and therefore can be included.
The patient is able to comply with scheduled visits, assessments and other study procedures.
The patient is willing to provide informed consent before enrolment in the study.

Exclusion Criteria:

The patient has previous or concomitant malignancies at other sites, except effectively treated non-melanoma skin cancers or carcinoma in situ of the cervix or effectively treated malignancy that has been in remission for over 5 years and highly likely to have been cured.
The patient has active hepatic encephalopathy at time of enrolment.
The patient has received a major organ allograft.
The patient is known to be positive for the Human Immunodeficiency Virus (HIV).
The patient has an uncontrolled bleeding disorder.
The patient has uncontrolled congestive heart failure or hypertension, unstable heart disease (coronary artery disease or myocardial infarction) or uncontrolled arrhythmia at the time of enrolment.
The patient has psychiatric or addictive disorders that may compromise his/her ability to give informed consent, or to comply with the study procedures.
The patient has other concurrent severe medical problems, unrelated to the malignancy, that would significantly limit full compliance with the study or expose the patient to unacceptable risk.
The patient has received any investigational or non-registered medicinal product (drug or vaccine) within the 30 days preceding the date of enrolment.
For female patients: the patient is pregnant or lactating.
The patient is unable to provide informed consent or refuse blood taking.
The patient has any other condition which, in the opinion of the Investigators, would make the patient unsuitable for enrolment or could interfere with completion of the study.

Contacts and Locations

Locations

	Site	City	Country	Postal Code
1	National University Hospital	Singapore	Singapore	119228
2	Singapore General Hospital	Singapore	Singapore	169608
3	National Cancer Centre Singapore	Singapore	Singapore	169610
4	Tan Tock Seng Hospital	Singapore	Singapore	308433
5	Changi General Hospital	Singapore	Singapore	529889
6	Sengkang General Hospital	Singapore	Singapore	544886