MMP-9 Expression in Permanent Mature Teeth With Symptomatic Pulpitis
Study Details
Study Description
Brief Summary
Matrix metallopeptidase-9 (MMP-9) expression was compared in healthy and inflamed pulp and the outcome of coronal pulpotomy in teeth with symptomatic pulpitis was assessed. After procedure blood samples were examined using Elisa kit.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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N/A |
Detailed Description
Introduction: Widely used diagnostic tests do not provide information to distinguish the pulp inflammation stage while improvement of materials, techniques and vital pulp therapy have received a wider acceptance in symptomatic pulpitis treatment. The aim of this clinical study was to compare MMP-9 expression in healthy and inflamed pulp and to assess the short-term outcome of coronal pulpotomy in mature permanent teeth with symptomatic pulpitis.
Methods: Patients diagnosed with symptomatic pulpitis were included in this clinical study. Coronal pulpotomy was performed using calcium-silicate based material and blood samples were taken. Initial and postoperative pain was recorded by Heft-Parker visual analog scale at 24 hours and 72 hours after the procedure. In control group teeth with healthy pulp were used. During follow-up visits after three and six-months, patients were examined clinically and radiographically. The ELISA kit was used to determine the levels of MMP-9 in inflamed and healthy coronal pulp tissue.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Other: Control group - Healthy teeth Patients with clinically healthy third molar or premolar teeth with indications for extraction; no clinical signs of pulpitis, no caries, no indications for the replacement of an old filling that is 1 mm from the pulp space as determined on the radiograph; a normal response to the cold test, and no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied. |
Procedure: Coronal pulpotomy
Coronal pulpotomy was performed with a sterile high-speed round bur under water coolant. Pulp vitality was confirmed by the presence of bleeding pulp tissue from all of the canal orifices. Hemostasis was achieved through the application of a pellet moistened with 2.5% NaOCl for two minutes and was repeated as needed. Calcium-silicate based material was prepared ac-cording to the manufacturer's instructions and gently placed over the pulp to fill the entire cavity.
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| Experimental: Test group - Teeth with pulpitis Patients with symptomatic pulpitis resulting from caries; tooth pain defined as sharp, dull, localized or diffuse; pain at night; a symptomatic tooth with a positive response to the cold test and lingering pain; intermittent or continuous episodes of spontaneous pain (with no external stimulus) that could last from a few minutes up to a few hours; no apical radiolucency on the radiograph. For teeth of this group coronal pulpotomy was applied. |
Procedure: Coronal pulpotomy
Coronal pulpotomy was performed with a sterile high-speed round bur under water coolant. Pulp vitality was confirmed by the presence of bleeding pulp tissue from all of the canal orifices. Hemostasis was achieved through the application of a pellet moistened with 2.5% NaOCl for two minutes and was repeated as needed. Calcium-silicate based material was prepared ac-cording to the manufacturer's instructions and gently placed over the pulp to fill the entire cavity.
|
Outcome Measures
Primary Outcome Measures
- MMP-9 amount measurement using ELISA kit [All samples were measured in one time (during 12 hours)]
The MMP-9 amount in the pulp blood samples was measured using the MMP-9 Human ELISA Kit. The measurement procedure was followed according to the manufacturer's instructions. The anti-MMP-9 polyclonal antibody was pre-coated on 96-well plates. Blood samples and a biotin-conjugated antibody were added to the wells supplemented with Avidin-Biotin-Peroxidase Complex and 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. A blue-colored product was produced and turned to yellow after an acidic stop solution was added. The intensity of the color yellow was proportional to the MMP-9 amount bound on the plate. The optical density absorbance was measured spectrophotometrically at 450nm in a microplate reader and the concentration of MMP-9 was calculated. For the expression of MMP-9 concentrations in the samples, a standard curve was used.
Eligibility Criteria
Criteria
Inclusion Criteria:
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Teeth diagnosed with symptomatic pulpitis
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Patients who did not use any NSAIDs before the treatment
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Positive cold test of investigated teeth
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Clinically dental caries in contact with pulp chamber
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Permanent teeth with radiographically closed root apex
Exclusion Criteria:
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Patients who refuse to participate in the study
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Medically compromised patients (with immunosuppressive/systemic diseases, patients on medications)
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Teeth with periapical radiolucency or clinical signs of apical periodontitis
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Periodontological compromised teeth (probing depth ≥4mm)
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Internal/external root resorption in periapical radiograph
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Pulp chamber and/or root canal calcification in periapical radiograph
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Teeth with unrestorable crown
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Teeth with a negative response to cold test
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Lithuanian University of Health Science | Kaunas | Lithuania |
Sponsors and Collaborators
- Eglė Gvazdaitytė
Investigators
- Study Director: Greta Lodiene, Lithuanian University of Health Science
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- BEC-LSMU(R)-01