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Release of Cytokines by Stimulated Peripheral Blood Mononuclear Cells (PBMC) From Chronic Periodontitis Subjects

Sponsor
Escola Bahiana de Medicina e Saude Publica (Other)
Overall Status
Completed
CT.gov ID
NCT00990041
Collaborator
School of Odontology of Piracicaba - Unicamp (Other)
1
Location

Study Details

Study Description

Brief Summary

Background and Objective: It has been reported that peripheral blood mononuclear cells (PBMC) from periodontal patients have a different profile of cytokine release when compared to the ones of healthy patients. Cigarette smoking is a recognized risk factor for periodontitis and is known to affect the systemic and local immune responses. Thus, the aim of the present study was to obtain preliminary data about the interaction of periodontal condition and smoking in the secretion of some cytokines by PBMC.

Material and Methods: PBMC samples were isolated from 19 donors, divided into generalized chronic periodontitis (P; n=10) and periodontal health (H; n=9) groups. In addition, the smokers and non-smokers of each group were separately evaluated: SP- smokers with severe generalized chronic periodontitis (n=5); SH- periodontally healthy smokers (n=5); NSP- non-smokers with severe generalized chronic periodontitis (n=5) and NSH- periodontally healthy non-smokers (n=4). Cells were incubated for 24-48 hours in 500 µL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of TNF-α, IL-6, IL-8 and IL-10 released using ELISA kits.

Condition or Disease Intervention/Treatment Phase

    Study Design

    Study Type:
    Observational
    Observational Model:
    Case-Control
    Time Perspective:
    Cross-Sectional

    Arms and Interventions

    Arm Intervention/Treatment
    PBMC
    periodontitis

    Outcome Measures

    Primary Outcome Measures

      Eligibility Criteria

      Criteria

      Ages Eligible for Study:
      25 Years to 55 Years
      Sexes Eligible for Study:
      All
      Accepts Healthy Volunteers:
      Yes
      Inclusion Criteria:
      • periodontitis
      Exclusion Criteria:
      • diabetes

      Contacts and Locations

      Locations

      Site City State Country Postal Code
      1 Faculdade de Odontologia de Piracicaba Piracicaba SP Brazil 13000

      Sponsors and Collaborators

      • Escola Bahiana de Medicina e Saude Publica
      • School of Odontology of Piracicaba - Unicamp

      Investigators

      None specified.

      Study Documents (Full-Text)

      None provided.

      More Information

      Publications

      None provided.
      Responsible Party:
      , ,
      ClinicalTrials.gov Identifier:
      NCT00990041
      Other Study ID Numbers:
      • 9002-10-04.PBMC
      First Posted:
      Oct 6, 2009
      Last Update Posted:
      Oct 6, 2009
      Last Verified:
      Oct 1, 2009
      Keywords provided by , ,
      periodontitis
      find cytokines in blood of periodontal patients
      Additional relevant MeSH terms:
      Periodontitis

      Study Results

      No Results Posted as of Oct 6, 2009