Role of Angiogenesis in Dermatologic Diseases: A Potential Therapeutic Target
Study Details
Study Description
Brief Summary
The researchers believe that pro-angiogenic factors are upregulated in a wide range of dermatologic diseases including port wine stains, hemangiomas, angiofibromas, Kaposi's sarcoma, angiosarcoma, scars, rosacea and psoriasis.
The researcher can perform immunohistochemistry and/or microarray analysis and/or quantitative polymerase chain reaction on previously biopsied skin specimens and newly biopsied skin specimens to evaluate the expression of various angiogenic factors in these dermatologic diseases.
In addition, some of the skin specimens may be utilized to make cell cultures to study expression of angiogenic factors and interactions of cells in dermatologic disease.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Detailed Description
Previously or newly collected biospecimens from various dermatologic diseases including port wine stains, hemangiomas, angiofibromas, Kaposi's sarcoma, angiosarcoma, scars, rosacea and psoriasis will be evaluated for markers of angiogenesis. Additionally, researchers can use discarded human skin tissue samples from skin biopsy/surgery sites which are removed for closure but are not submitted for histopathologic analysis.
Currently, biospecimens prospectively collected are only from lesions with a known diagnosis:
- port wine stains, hemangiomas, cherry angiomas, facial angiofibromas, scars and psoriasis these lesions are generally not biopsied for diagnosis or 2) previously biopsied and diagnosed other vascular lesions such as angiosarcomas and Kaposi's Sarcoma.
Specimens collected will be processed for microarray analysis, qPCR and/or immunohistochemistry performed to evaluate expression of various angiogenic factors and their receptors including: vascular endothelial growth factor, basic fibroblast growth factor, angiopoietin 1, angiopoietin 2, matrix metalloproteinase, tissue inhibitor metalloproteinase I and thrombospondin-1, Angiotensin 2 receptor.
Additional, tissue samples will be used to isolate three cell types: endothelial cells, keratinocytes, and fibroblasts. Tissue samples will be digested to isolate the cells which will be cultured separately and then incorporated into an in-vitro model to observe how blood vessels form in skin affected by port wine stains as compared to vessel growth in unaffected skin.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| dermatologic diseases skin tissue sample |
Other: skin tissue sample
skin tissue sample
|
Outcome Measures
Primary Outcome Measures
- Factors in dermatologic diseases. [up to 4 weeks]
Evaluate the role of angiogenesis in cutaneous disease and ultimately, facilitate implementation of anti-angiogenic therapy in a wide range of dermatologic diseases including port wine stains, hemangiomas, angiofibromas, Kaposi's sarcoma, angiosarcoma, scars, rosacea and psoriasis.
Eligibility Criteria
Criteria
Inclusion Criteria:
-
18 years of age or older.
-
willing to have a skin biopsy
Exclusion Criteria:
-
under 18 years of age
-
unable to carry out instructions
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Beckman Laser Institute Medical clinic | Irvine | California | United States | 92612 |
| 2 | Gottschalk Medical Plaza | Irvine | California | United States | 92612 |
Sponsors and Collaborators
- University of California, Irvine
- Beckman Laser Institute University of California Irvine
Investigators
- Principal Investigator: Kristen Kelly, MD, Beckman Laser Institute University of California Irvine
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- NIH/LAMMP-2007-6094