Serum Folic Acid Status, Genotyping MTHFR 677C-->T, Plasma Homocysteine, DNA Methylation of IGF2 Gene and Microbiota of the Children: Mother-Children Cohort Study
Study Details
Study Description
Brief Summary
This study is part of the East Jakarta Cohort Study to assess the current micronutrient status of the mothers and their children. The study also includes the assessment of genotyping of MTHFR 677C-->T of the mother and DNA methylation of IGF2 gene of the children.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
The initial study recruited 315 mothers in the public health centre and one district hospital in East Jakarta. The follow-up study is able to re-visit the 127 of them. This also includes 127 peers of the subject that lives in the same neighbourhood. The design of the study is case-cohort design, part study of the existing cohort. The assessment of the follow-up study includes the mother and the children born in the initial study. Anthropometric measurement, socioeconomic data from a structured questionnaire, biomarker assessment, blood pressure were obtained from the subjects.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| East Jakarta Cohort Study One group included in the study was originated from mothers who participated in the first data collection pregnancy. The follow-up study included mothers and their children born as the subject in the follow-up in the study. This study also includes peer of the subjects that lived in the same neighbourhood since the first cohort study and have children in the same age with cohort participant |
Outcome Measures
Primary Outcome Measures
- Serum folic acid status [December 2018 - March 2019]
The measurement of serum folic acid status is using LC-MS/MS
- Genotyping MTHFR 677C-->T [December 2018 - March 2019]
The assessment of MTHFR 677C-->T using Real Time PCR
- DNA methylation of IGF2 gene [December 2018 - March 2019]
DNA methylation of IGF2 gene using pyrosequencing method
Secondary Outcome Measures
- Serum Homocysteine [December 2018-March 2019]
The measurement of serum homocysteine using Electrochemiluminescen
- Serum Adiponectin [December 2018-March 2019]
The measurement of serum adiponection using electrochemiluminescence
- Blood pressure [December 2018-March 2019]
The blood pressure measurement using semi automatic device (Omron HBP1300)
Other Outcome Measures
- Nutritional status assessment [September 2018-December 2018]
Using antrhopometric measurement, using SECA for weight measurement, Shorrboard for height and standardized plastic tape for mid upper arm circumference.
Eligibility Criteria
Criteria
Inclusion Criteria:
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participant in the previous study
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apparently healthy
-
data complete
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voluntarily to participate
Exclusion Criteria:
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not complete data
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migrate
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refuse to participate in the study
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Universitas Indonesia | Jakarta | DKI Jakarta | Indonesia | 10430 |
Sponsors and Collaborators
- Indonesia University
- Ministry of Research and Higher Education of Republic Indonesia
- United States Agency for International Development (USAID)
Investigators
- Study Director: Rina RA Agustina, Doctoral, Indonesia University
Study Documents (Full-Text)
None provided.More Information
Additional Information:
- The effect of folic acid supplementation on fetus growth and development
- The maternal folic acid supplementation results strengthen the link between folic acid supplementation during later pregnancy and epigenetic changes and identify a novel mechanism for regulation of ZFP57.
Publications
None provided.- 0420/UN2.F1/ETIK/2018