Evaluation of Serum Interleukin 6 and 8 in Children With Acute Gastroenteritis
Study Details
Study Description
Brief Summary
Cytokines, such as IL-6 and IL-8 can be used as markers of acute infections, including acute gastroenteritis. This study aims to evaluate serum levels of interleukins 6 and 8 in children with acute gastroenteritis.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Cases Children with acute diarrhea |
Diagnostic Test: Evaluation of serum levels of IL-6 and IL-8
Evaluation of serum levels of IL-6 and IL-8 by Luminex Assay (multiplexed ELISA kits)
|
| Control Healthy control children |
Diagnostic Test: Evaluation of serum levels of IL-6 and IL-8
Evaluation of serum levels of IL-6 and IL-8 by Luminex Assay (multiplexed ELISA kits)
|
Outcome Measures
Primary Outcome Measures
- Serum level of Interleukin 6 [Within the first 3 days of acute diarrhea]
Difference in serum level of interleukin 6 between cases and control groups
- Serum level of Interleukin 8 [Within the first 3 days of acute diarrhea]
Difference in serum level of interleukin 8 between cases and control groups
Eligibility Criteria
Criteria
Group 1: Cases (Children with acute diarrhea)
Inclusion Criteria:
-
Children aged 6 months to 5 years.
-
Acute diarrhea: defined according to WHO criteria as the "passage of loose or watery stools at least three times in a 24 h period", lasts less than 14 days, with or without fever or vomiting, but considering the importance of change in stool consistency rather than frequency, and the usefulness of parental insight in deciding whether children have diarrhea or not.
Exclusion Criteria:
-
Malnutrition or obesity
-
History of antibiotic therapy in the last 72 hours.
-
Acute diarrhea in association with systemic infections
-
Malignancy
-
Chronic diarrhea
-
Autoimmune and autoinflammatory diseases.
-
Chronic renal/liver failure
-
Diabetes mellitus
Group 2: Controls (Healthy control children)
Inclusion Criteria:
• Healthy children aged 6 months to 5 years.
Exclusion Criteria:
-
Acute diarrhea
-
Malnutrition or obesity
-
History of antibiotic therapy in the last 72 hours.
-
Acute diarrhea in association with systemic infections
-
Malignancy
-
Chronic diarrhea
-
Autoimmune and autoinflammatory diseases.
-
Chronic renal/liver failure
-
Diabetes mellitus
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Sohag University Hospital | Sohag | Egypt |
Sponsors and Collaborators
- Sohag University
Investigators
None specified.Study Documents (Full-Text)
None provided.More Information
Publications
- Chen SM, Lin CP, Tsai JD, Chao YH, Sheu JN. The significance of serum and fecal levels of interleukin-6 and interleukin-8 in hospitalized children with acute rotavirus and norovirus gastroenteritis. Pediatr Neonatol. 2014 Apr;55(2):120-6. doi: 10.1016/j.pedneo.2013.05.008. Epub 2013 Jul 27.
- Hamiel U, Bahat H, Kozer E, Hamiel Y, Ziv-Baran T, Goldman M. Diagnostic markers of acute infections in infants aged 1 week to 3 months: a retrospective cohort study. BMJ Open. 2018 Jan 24;8(1):e018092. doi: 10.1136/bmjopen-2017-018092.
- Muhsen K, Kassem E, Rubenstein U, Goren S, Ephros M, Shulman LM, Cohen D. No evidence of an increase in the incidence of norovirus gastroenteritis hospitalizations in young children after the introduction of universal rotavirus immunization in Israel. Hum Vaccin Immunother. 2019;15(6):1284-1293. doi: 10.1080/21645515.2019.1599522. Epub 2019 May 3.
- Soh-Med-21-12-07