Evaluation of Platelet Rich Plasma in Skin Graft Take for Patients With Post Burn Raw Areas
Study Details
Study Description
Brief Summary
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To evaluate the effect of the platelet rich plasma on the take of split thickness skin graft and its early and late results in resurfacing of post burn raw areas.
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To evaluate the effect of PRP on wound healing on skin graft donor site.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
|
N/A |
Detailed Description
Burn injuries involve tissue destruction due to energy transfer,The burn injuries can be caused by heat from hot liquids, solids or fire, an less common causes are friction, heat, chemical or electric sources The treatment of burns is complex due to the combination of hypermetabolic physiological response and the need for good functional and cosmetic recovery. Split thickness skin grafts (STSGs) are commonly used to resurface large areas of Post burn raw areas.
The time to complete wound healing ranged from 2 to 75 days. Factors that significantly affected time to complete wound healing were graft loss by seroma, presence of hypergranulation tissue, premorbid conditions, and total body surface area burned.
Generally, skin graft undertakes three steps for healing: anchorage, inosculation, and maturation. Haematoma, shearing, and infection on recipient sites of skin graft can reduce skin graft take, and even lead to skin graft loss.
Platelet-rich plasma (PRP) is an autologous blood-derived biomaterial that is enriched with a 2- to 6-fold concentration of platelets in comparison to normal human platelet concentration. Multiple growth factors and bioactive proteins are stored in α-granules of platelets. These growth factors can promote proliferation of endothelial cells, epithelial cells as well as fibroblasts, and increase angiogenesis. The potential clinical application of PRP in skin graft might rely on its haemostasis, adhesive, antibacterial and healing properties.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| Experimental: group with PRP Group of patients with post burn raw areas treated with application of platelet rich plasma |
Procedure: PRP in skin grafts
topical application of platelet rich plasma before resurfacing with STSG in Post burn raw areas
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| Experimental: group without PRP Group of patients with post burn raw areas treated with conventional methods |
Procedure: conventional methods in skin grafts
fixation of the graft with stitches and staplers
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Outcome Measures
Primary Outcome Measures
- histopathological biopsy from both groups by punch Biopsy (vancouver scale ) [3 months]
pigmentation (0-2) ,normal 0, Hypopigmentation 1 ,Hyperpigmentation 2 Vascularity (0-3) ,Normal 0, Pink 1, Red 2, Purple 3 Pliability (0-5) Normal 0 ,Supple 1 ,Yielding 2,Firm 3, Banding 4, Contracture 5 Height (0-3) Normal(flat)0 ,(0-2mm)1,(2-5mm)2,(>5mm)3
Eligibility Criteria
Criteria
Inclusion Criteria:
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Age (15- 60 years).
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Post burn Raw area less than 10 % of total body surface area .
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Selected donor sites.
Exclusion Criteria:
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Raw area due to other causes ( trauma, ischemia,..etc)
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Patients with bleeding tendency.
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Patients with comorbidities(diabetes, malignancy,..etc)
Contacts and Locations
Locations
No locations specified.Sponsors and Collaborators
- Assiut University
Investigators
- Study Chair: Mostafa Elsonbaty, prof, Assiut University
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- PRP in skin grafts