Targeted Metabolomics and Spent Embryo Culture Medium
Study Details
Study Description
Brief Summary
More than 8 million babies have been born through in vitro fertilization (IVF). Non-invasive observation of embryos in vitro to better understand their development is becoming increasingly important. Morphology has been used as standard from the beginning, but has the disadvantage of subjectivity. Now the emphasis in basic and clinical research is on developing rapid, quantitative, non-invasive tests. Hence comes the idea of metabolic profiling of spent embryo culture medium (SECM) as a biomarker. This could be useful for understanding and improving the nutritional environment of oocytes and embryos. The goal of our study is to determine metabolic profiles of the SECM in combination with morphological assessments to better understand the nutritional requirements of the embryo. The goal would be to optimize media specifically, depending on patient and embryo characteristics ("personalized medicine") ("the embryo in vitro as patient").
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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Detailed Description
Following successful fertilization (day 1), each embryo will be cultured for 5-6 days (day 5-6) to form the blastocyst. Then, morphological evaluation will be performed by an expert embryologist to confirm poor and good embryo quality. At the end of the culture, 50 µl of SECM will be collected from each embryo and stored for metabolomics analysis. Samples including medium only and no embryos will be used as controls. The metabolome will be characterized in SECM samples using the respective assay targeted kit.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| good or poor embryo quality morphological evaluation will be performed by an expert embryologist to confirm poor and good embryo quality |
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| metabolomics profiles metabolomics profiles will be performed by a Metabolomics core facility |
Outcome Measures
Primary Outcome Measures
- Metabolomics profiles of good vs. Metabolomics profiles of poor quality embryos [24 months]
Metabolomics profiles
Secondary Outcome Measures
- amino acids profiles of good vs. amino acids profiles of poor quality embryos [24 months]
amino acids profiles
Eligibility Criteria
Criteria
Inclusion Criteria:
- Available spent embryo culture medium (SECM) samples with Informed consent
Exclusion Criteria:
- Diabetes
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Gyn-Medicum | Göttingen | Niedersachsen | Germany | 37073 |
Sponsors and Collaborators
- gyn-medicum Göttingen
Investigators
- Study Chair: Andreas Schmutzler, PD Dr. med., gyn-medicum Göttingen
Study Documents (Full-Text)
None provided.More Information
Publications
- Cimadomo D, Rienzi L, Conforti A, Forman E, Canosa S, Innocenti F, Poli M, Hynes J, Gemmell L, Vaiarelli A, Alviggi C, Ubaldi FM, Capalbo A. Opening the black box: why do euploid blastocysts fail to implant? A systematic review and meta-analysis. Hum Reprod Update. 2023 Sep 5;29(5):570-633. doi: 10.1093/humupd/dmad010.
- Inoue N, Nishida Y, Harada E, Sakai K, Narahara H. GC-MS/MS analysis of metabolites derived from a single human blastocyst. Metabolomics. 2021 Jan 25;17(2):17. doi: 10.1007/s11306-021-01770-x.
- Siristatidis C, Dafopoulos K, Papapanou M, Stavros S, Pouliakis A, Eleftheriades A, Sidiropoulou T, Vlahos N. Why Has Metabolomics So Far Not Managed to Efficiently Contribute to the Improvement of Assisted Reproduction Outcomes? The Answer through a Review of the Best Available Current Evidence. Diagnostics (Basel). 2021 Sep 2;11(9):1602. doi: 10.3390/diagnostics11091602.
- gyn-medicumGöttingen