Urinary Proteomics Analysis for Sepsis and Prognosis
Study Details
Study Description
Brief Summary
As a noninvasive examination, urinary proteomics is a very useful tool to identify renal disease. The purpose of the present study was to find differential proteins among patient with SIRS and sepsis(included survivors and non-survivors), and to screen potential biomarkers for the early diagnosis of sepsis and its prognosis. Urinary proteins were identified by iTRAQ labeling and LC-MS/MS. The bioinformatics analysis was performed with the Mascot software and the International Protein Index (IPI) and the Gene Ontology (GO) Database and KEGG pathway Database. The differentially expressed proteins were verified by Western blot by another sample collected from clinical.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| SIRS temperature >38 ℃ or <36℃; pulse rate>90 beats/min; ventilatory rate>20 breaths/min or hyperventilation with partial pressure of arterial carbon dioxide (PaCO2)<32mmHg; white blood cell count>12,000μL-1 or <4000μL-1 or >10% immature cells |
|
| sepsis Sepsis sepsis: SIRS plus infection; severe sepsis: sepsis associated with organ dysfunction, hypoperfusion, or hypotension; septic shock: sepsis with arterial hypotension, despite adequate fluid resuscitation. |
|
| non-survivors with sepsis sepsis patients who died within 28 days |
Outcome Measures
Primary Outcome Measures
- Survival status [28days after admited in ICU]
The survival time of patients more than 28days is defined as survival. The survival time of patients less than 28days is defined as death
Eligibility Criteria
Criteria
Inclusion Criteria:
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Male and female aged 18 years old and over;
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clinically confirmed infection;
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fulfilled at least two criteria of systemic inflammatory response syndrome
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(a) core temperature higher than 38 °C or lower than 36 °C
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(b)respiratory rate above 20/min, or PCO2 below 32 mmHg
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(c) pulse rate above 90/min, and
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(d) white blood cell count greater than 12,000/μl or lower than < 4,000/μl or less than 10% of bands.
Exclusion Criteria:
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younger than 18 years of age;
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acquired immunodeficiency syndrome;
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reduced polymorphonuclear granulocyte counts (< 500 μL-1);
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died within 24h after admission into the ICU, or refused to participate in the study, or declined treatment during the period of observation.
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Chinese PLA General Hospital | Beijing | Beijing | China | 100853 |
Sponsors and Collaborators
- Chinese PLA General Hospital
Investigators
- Study Director: Lixin Xie, MD, Department of Respiratory Diseases, Chinese PLA General Hospital
Study Documents (Full-Text)
None provided.More Information
Publications
None provided.- 20111013-007(1)
- 2009BAI86B03