In Vitro Maturation Induction of Oocytes From a Woman With Syndrome of Repeated Oocyte Maturation Failure in Vitro

• Sponsor: The Second Affiliated Hospital of Kunming Medical University (Other)
• Overall Status: Completed
• CT.gov ID: NCT01977664
• Collaborator: (none)
• Enrollment: 1
• Location: 1
• Duration (Months): 1
• Patients Per Site Per Month: 1

Study Details

Study Description

Brief Summary

Donated oocytes is from a patient with repeated oocyte maturation failure in IVF cycles. Cytoplasm of matured metaphase II oocyte and active MPF were injected into the donated oocytes.

Condition or Disease	Intervention/Treatment	Phase
Oocyte Maturation Failure	Other: no intervention

Detailed Description

The study reported a woman with syndrome of repeated oocyte maturation failure where there is repeated production of immature oocytes.She underwent two IVF cycles in other hospital. All oocytes obtained were immature and the treatment were stopped.She underwent her third try in our hospital. The couple gave their informed consent: ICSI will be performed if maturation occurred, otherwise, the treatment would be stopped and the oocytes would be donated for study if the oocytes were not matured after 24 hours of culture. After ovarian hyperstimulation,14 oocytes were obtained. None of them was matured. The cycle was canceled and the oocytes were donated for study.To induce the immature oocytes in vitro, oocytes were 1)cultured in follicular fluid containing matured metaphase II oocyte; 2) performed intracytoplasmic sperm injection (ICSI) to induce intracellular Ca2+]i oscillations; 3)injected with active maturation promoting factor (MPF), 4)injected with cytoplasm of matured metaphase II oocyte.

Study Design

• Study Type: Observational
• Actual Enrollment: 1 participants
• Observational Model: Case-Only
• Time Perspective: Cross-Sectional
• Official Title: The Factors Primarily Intrinsic to the Oocyte Are Responsible to the Oocyte Maturation Defective: a Case Report.
• Study Start Date: Mar 1, 2012
• Actual Primary Completion Date: Apr 1, 2012
• Actual Study Completion Date: Apr 1, 2012

Arms and Interventions

Arm	Intervention/Treatment
oocyte maturation failure no intervention	Other: no intervention

Outcome Measures

Primary Outcome Measures

1. Oocyte nucleus maturation [April，2012]

Eligibility Criteria

Criteria

• Ages Eligible for Study: 32 Years to 32 Years
• Sexes Eligible for Study: Female
• Accepts Healthy Volunteers: No

Inclusion Criteria:

Clinical diagnosis of syndrome of repeated oocyte maturation

Exclusion Criteria:

patients with matured oocytes in IVF cycles

Contacts and Locations

Locations

	Site	City	State	Country	Postal Code
1	The Second Affiliated Hospital of Kunming Medical University	Kunming	Yunnan	China	650101

Sponsors and Collaborators

• The Second Affiliated Hospital of Kunming Medical University

Investigators

• Principal Investigator: Jie Yang, M.Sc, The Second Affiliated Hospital of Kunming Medical University

Study Documents (Full-Text)

None provided.

More Information

Publications

None provided.

• Responsible Party: Yang Jie, Principal Investigator, The Second Affiliated Hospital of Kunming Medical University
• ClinicalTrials.gov Identifier: NCT01977664
• Other Study ID Numbers: 201301
• First Posted: Nov 7, 2013
• Last Update Posted: Nov 7, 2013
• Last Verified: Oct 1, 2013

• Keywords provided by Yang Jie, Principal Investigator, The Second Affiliated Hospital of Kunming Medical University: oocyte maturation failure; Maturation induction in vitro

Study Results

No Results Posted as of Nov 7, 2013