Compare the Efficacy of the Remineralization Agents on the White Enamel Lesions
Study Details
Study Description
Brief Summary
The purpose of this study is to determine whether Fluoride varnish + calcium (Clinpro White Varnish) and polipeptide solution (Curodont Repair) are effective in the treatment of remineralization therapy. The investigators will diagnose their alone or in combination effects on white spot lesions. Aesthetics (tooth-colored), remineralization, microbiological effects will be evaluated separately. Spectrophotometer (Vita Easy Shade) , Quantitative Light Fluorescence (QLF), Diagnodent(Cavo Diagnodent) and culture methods in microbiology laboratories are used for aesthetic, remineralization and microbiological assessments. The measurements will be made baseline, 6 weeks, 3 months and 6 months later after the applications and recorded.
| Condition or Disease | Intervention/Treatment | Phase |
|---|---|---|
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N/A |
Detailed Description
The aim of this study is that evaluate the remineralizing and antibacterial effects of the different remineralization agents. Initiative caries lesions named as white spot lesions and these lesions are localized at enamel. Demineralization is occured but cavitations aren't seen at this stage. It is important to control at this stage with remineralization agents because of the minimally invasive approach.
Fluoride varnish or polipeptide containing agents alone or combination using effects on remineralization, esthetic and bacterial counts will be evaluated with Quantitive Light Fluorescence , Diagnodent (Cavo-Diagnodent), Spectrophotometer (Vita Easy Shade) devices and microbiological assessment at laboratory.
The investigators will choose and include in our study that who come to the Ege University Faculty of Dentistry pediatric department, between 10-18 years olds, healthy and have one or more white spot lesions on her/his permanent teeth.
32 volunteer who have inclusion criteria will be put into pratice. 32 volunteer divided into 4 groups for different applications. We will tell each volunteer that they must brush their teeth twice a day with fluoride dentifrice ( the investigators will give them 1400 ppm fluoride containing fluoride dentifrice)
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Group: Control group ( The investigators won't do any application , the oral hygiene konwledge will be given only.)
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Group: Fluoride + Calcium (TCP) (The investigators will do fluoride+ calcium varnish( Clinpro white varnish) application on white spot lesions)
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Group: Polipeptide solution (The investigators will do polipeptide ( Curodont repair) solution application on white spot lesions)
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Group: [Fluoride + calcium varnish] + polipeptide solution combination ( The investigators will do polipeptide ( Curodont repair) solution and fluoride + calcium varnish (Clinpro white varnish) application on white spot lesions) First day,the investigators will collect stimulated saliva from each volunteer before any application. Saliva samples will transport to the microbiology laboratory. After any application 6 weeks, 3 months and 6 months later we will collect stimulated saliva again for microbiological assessment.
Nyvad Caries Diagnostic Criteria , ICDAS indexes will be used to make the follow-up of lesions at baseline and 6 months after the application. Plaque index (Silness & Löe , 1964) and bleeding index (Ainamo&Bay ,1976) will be used to assess oral hygiene every visit.
The investigators will use QLF,Diagnodent devices will be used at beginning for evaluate the demineralization level and after applications 6 weeks, 3 months and 6 months later used for evaluate the remineralization level. In addition for determine about esthetic results the investigators will use spectrophotometer (Vita Easy Shade) device at beginning, 6 weeks, 3 months and 6 months later.
Study Design
Arms and Interventions
| Arm | Intervention/Treatment |
|---|---|
| No Intervention: Control The investigators won't do any application , the oral hygiene konwledge will be given only. |
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| Active Comparator: Fluoride + Calcium varnish The investigators will do fluoride+ calcium varnish( Clinpro White Varnish) application on white spot lesions. |
Other: Clinpro White Varnish (fluoride + calcium varnish)
Clinpro White Varnish will be applied to the teeth with brush.
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| Experimental: Polipeptide solution The investigators will do polipeptide ( Curodont Repair) solution application on white spot lesions. |
Other: Curodont Repair
Curodont Repair solution be applied to the teeth in the form of drops
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| Experimental: Fluoride + calcium + polipeptide The investigators will do polipeptide ( Curodont Repair) solution and fluoride + calcium varnish (Clinpro White Varnish) application on white spot lesions. |
Other: Clinpro White Varnish (fluoride + calcium varnish)
Clinpro White Varnish will be applied to the teeth with brush.
Other: Curodont Repair
Curodont Repair solution be applied to the teeth in the form of drops
|
Outcome Measures
Primary Outcome Measures
- QLF ( ∆F, ∆Q, lesion area) [Change baseline enamel fluorescence level at 6 weeks, 3 and 6 months.]
remineralization level assesment
Secondary Outcome Measures
- Salivary Streptococcus mutans and lactobacilli counts [baseline, after 6 weeks, 3 months and 6 months of the application]
Microbiological assesment
- Spectrophotometer ( L, a, b) [baseline, after 6 weeks, 3 months and 6 months of the application]
esthetic assesment
- Diagnodent [baseline]
remineralization level assesment
- Nyvad Caries Diagnostic Criteria index and ICDAS index [baseline]
Evaluation of the state of the lesion
Eligibility Criteria
Criteria
Inclusion Criteria:
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between 10-18 years olds
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healthy
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have one or more white spot lesions on her/his permanent teeth
Exclusion Criteria:
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have any health problem
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unsuitable age
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don't give approval for this study
Contacts and Locations
Locations
| Site | City | State | Country | Postal Code | |
|---|---|---|---|---|---|
| 1 | Ege University School of Dentistry Department of Pedodontics | İzmir | Turkey |
Sponsors and Collaborators
- Ege University
Investigators
- Principal Investigator: Ecem Ergin, Ege University
- Study Director: Ece Eden, Ege University
- Study Chair: Mustafa Ateş, Ege University
Study Documents (Full-Text)
None provided.More Information
Publications
- Beerens MW, Boekitwetan F, van der Veen MH, ten Cate JM. White spot lesions after orthodontic treatment assessed by clinical photographs and by quantitative light-induced fluorescence imaging; a retrospective study. Acta Odontol Scand. 2015 Aug;73(6):441-6. doi: 10.3109/00016357.2014.980846. Epub 2014 Nov 25.
- Gomez J, Pretty IA, Santarpia RP 3rd, Cantore B, Rege A, Petrou I, Ellwood RP. Quantitative light-induced fluorescence to measure enamel remineralization in vitro. Caries Res. 2014;48(3):223-7. doi: 10.1159/000354655. Epub 2014 Jan 29.
- Kirkham J, Firth A, Vernals D, Boden N, Robinson C, Shore RC, Brookes SJ, Aggeli A. Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res. 2007 May;86(5):426-30.
- Lelli M, Putignano A, Marchetti M, Foltran I, Mangani F, Procaccini M, Roveri N, Orsini G. Remineralization and repair of enamel surface by biomimetic Zn-carbonate hydroxyapatite containing toothpaste: a comparative in vivo study. Front Physiol. 2014 Sep 5;5:333. doi: 10.3389/fphys.2014.00333. eCollection 2014.
- Mehta R, Nandlal B, Prashanth S. Comparative evaluation of remineralization potential of casein phosphopeptide-amorphous calcium phosphate and casein phosphopeptide-amorphous calcium phosphate fluoride on artificial enamel white spot lesion: an in vitro light fluorescence study. Indian J Dent Res. 2013 Nov-Dec;24(6):681-9. doi: 10.4103/0970-9290.127610.
- Oliveira GM, Ritter AV, Heymann HO, Swift E Jr, Donovan T, Brock G, Wright T. Remineralization effect of CPP-ACP and fluoride for white spot lesions in vitro. J Dent. 2014 Dec;42(12):1592-602. doi: 10.1016/j.jdent.2014.09.004. Epub 2014 Sep 23.
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